Anti-Phospho-elF-2α (Ser51) Rabbit Monoclonal Antibody Data Sheet RM298
Specificity
RM298 reacts to human eIF-2α (eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. There is no cross-reactivity to eIF-2α not phosphorylated at Ser51. This antibody may also react to mouse or rat Phospho-eIF-2α (Ser51), as predicted by immunogen homology.
Immunogen
A phospho-peptide corresponding to human Phospho- eIF-2α (Ser51)
Application
Immunohistochemistry (IHC)
Western Blot (WB)
Species Reactivity
Human
Conjugate
None
Intended Use
Research Use Only
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Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size
100 uL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
Clone Type
Monoclonal
Isotype
Rabbit IgG
Usage
Immunohistochemistry (IHC): 1:100-1:200 dilution;
Western Blot (WB): 1:100-1:200 dilution.
Keywords
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Product Images
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-phospho-eIF-2α (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid cancer tissue section using anti-phospho-eIF-2α (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.
Western Blot of HeLa cell lysate nontreated (-) or treated (+) with Calyculin A (CalA), using anti- phospho-eIF-2α (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.