Western blot of nonreduced(-) and reduced(+) mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mL of RevMAb clone RM110. This antibody reacts to nonreduced IgG1λ.
ELISA of mouse immunoglobulins shows RM110 reacts to the lambda light chain of mouse immuno- globulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno- globulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM110 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG1λ. The plate was coated with different amounts of mouse IgG1λ. A serial dilution of RM110 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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